Enzymes and Inhibitors
Sigma Aldrich Fine Chemicals Biosciences Hyaluronidase from bovine testes | 37326-33-3 | MFCD00131351 | 5 g
Hyaluronidase from bovine testes | Mol Wt: ~55 kDa | 37326-33-3 | MFCD00131351 | 5 g
Sigma Aldrich Fine Chemicals Biosciences Apyrase from potatoes, 9000-95-7, MFCD00130542, 500UN
Suitable for manufacturing of diagnostic kits and reagents, ATPase ≥200 units/mg protein. Synonym: Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase, Apyrase from potatoes. Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation.
Sigma Aldrich Fine Chemicals Biosciences Pectinase from Aspergillus aculeatus | 50 mL
Pectinase is an enzyme from Aspergillus aculeatus that is used in plant protoplast preparation to digest cell wall prior to organelle isolation. It has been used to conduct partial saccharification of sugars. Pectinases are used to study their role in the invasion of plant tissues by phytopathogens, the spoilage of produce and various food processing and plant biotechnology applications. Pectinase, product P2611, has been used to treat leaf discs from fifth wild-type and sitiens leaves.
Sigma Aldrich Fine Chemicals Biosciences beta-Glucuronidase from limpets (Patella vulgata) aqueous solution | 9001-45-0 | MFCD00130629 | 10ML
beta-Glucuronidase from limpets (Patella vulgata) aqueous solution | 9001-45-0 | MFCD00130629 | 10ML
New England Biolabs, Inc. NsiI-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence ATGCA^T. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. DraIII-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence CAC_NNN^GTG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. SphI-HF – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence GCATG^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
QIAGEN INC BUFFER AW2
NC2406403 BUFFER AW2
Sigma Aldrich Fine Chemicals Biosciences Pyruvate Kinase from rabbi
Pyruvate kinase from rabbit muscle catalyzes an ATP-dependent phosphorylation of glycolate to yield 2-phosphoglycolate.
AdipoGen VX-745
Chemical. CAS 209410-46-8. Formula C19H9Cl2F2N3OS. MW 436.3. VX-745 is a potent inhibitor of p38α MAPK IC50=10nM with 20-fold selectivity over p38β and 1000-fold selectivity over closely related kinases, ERK1, MK2 and JNK1-3. p38α is a member of the mitogen-activated protein kinase MAPK family of serine/threonine protein kinases and is widely expressed in endothelial, immune or inflammatory cells and plays a central role in the regulation of proinflammatory cytokine production including TNF-α, IL-1β and IL-6. VX-745 is an anti-inflammatory agent that blocks IL-1β and TNF-α release in whole blood in vitro with IC50 values of 150 and 180nM, respectively. VX-745 showed anti-inflammatory efficacy in an animal model of rheumatoid arthritis and recently anti-neuroinflammatory potential in prevention of early-stage Alzheimers diseases AD.
Alkali Scientific Proteinase K, 25 Mg
Proteinase K is a highly active protease enzyme commonly used in molecular biology applications, particularly for DNA and RNA extraction. It catalyzes the breakdown of proteins, allowing for the efficient release of nucleic acids from cellular structures during lysis. With its high activity and broad specificity, Proteinase K is effective even in the presence of detergents and other inhibitors commonly used in DNA extraction protocols. Available in a 25 mg package, this reagent is ideal for small-scale experiments or specific laboratory applications where protein degradation is required. Proteinase K is a key enzyme in high-efficiency nucleic acid isolation protocols and enzyme inactivation procedures.
Medchemexpress LLC Pyruvate Kinase, Microorganism | 9001-59-6 | 237 kDa | 1 KU
Pyruvate Kinase, Microorganism (PK) is a glycolytic enzyme that catalyzes the conversion of phosphoenolpyruvate and ADP to pyruvate and ATP. This product is for research use only.
- Catalyzes conversion of phosphoenolpyruvate and ADP to pyruvate and ATP
- Theoretical molecular weight: 237 kDa
- Optimum pH: 7.0-7.5
- Appears as a white to off-white solid
- For research use only
Revvity Health Sciences Inc 14C & 3H Extended Range Quench Standard Set, 20 mL Vial
14C & 3H Extended Range Quench Standard Set, 20 mL Vial
Medchemexpress LLC Pyruvate kinase (PK), rabbit muscle (ATP:pyruvate 2-0-phosphotransferase, PK) | 9001-59-6 | 5 KU
Pyruvate kinase (PK), rabbit muscle (ATP:pyruvate 2-0-phosphotransferase, PK) is a biological material or organic compound suitable for life science research.
- Solid appearance
- White to off-white color
- EC number 2.7.1.40
- Specific activity 350-600 U/mg protein
- One unit converts 1 μmol of phospho(enol)pyruvate to pyruvate per minute at pH 7.6 at 37°C
- For research use only